ブックタイトルH25博士学位論文_全ページ

概要

H25博士学位論文_全ページ

various herbs. The contact hypersensitivity (CHS) in mice is commonly used to evaluatethe anti-allergic activity of herbal medicines. In the present study, the hot water extract ofA. camansi leaves showed the most significant inhibitory effect on 2, 4, 6-trinitrochlorobenzene (TNCB)-induced contact hypersensitivity. The ear swelling 24-48 hafter the challenge of the A. camansi extract treated mice was significantly less than that ofthe mice that received hot water extract of F. pseudopalma and P. odorata leaves. Thesame responses were observed from the mice that received the kamansi ethanol-precipitate(KEP) and kamansi ethanol-precipitate water-soluble (KEPWS) fractions. Since the highmolecular mass fraction showed the significant activity, we speculated that the activecompound responsible might be a polysaccharide and/or glycoprotein.The results we obtained from different in vivo and in vitro analyses in this studyshow some of the pharmacological activities of F. pseudopalma, A. camansi, and P.odorata related to their traditional and folkloric use, and it hopes to contribute to theThus, the search for alternative and safer herbal medicines is still of great interest. In our invitro studies, the hot water extract from the leaves of P. odorata and A. camansi hadsignificant inhibitory activity against ??-glucosidase. The modes of inhibitions of the plantswere also demonstrated. The extract of P. odorata showed a competitive inhibition whilethat of A. camansi exhibited non-competitive inhibition. Moreover, further fractionation ofthe P. odorata extract revealed a more potent enzyme inhibitor than acarbose. Highperformanceliquid chromatography (HPLC), 1H- and 13C-Nuclear magnetic resonance(NMR) analyses confirmed that the fraction with the highest inhibitory activity containsActeoside.Medicinal plants with reported anti-inflammatory activity could have the potentialuse as anti-allergens and inhibitors of ACD reactions produced by allergens and chemicals.In the past few years, much research had focused on the immunomodulatory effects of－５３－